Phosphinothricin ppt
WebPhosphinothricin (PPT) is one of the most prevalently using herbicides. The commercial phosphinothricin products are generally in the form of a racemic mixture, of which only … Web168 protocolsare often genotypespecific (Widholm, 1995; Somers et al., 2003). Increased soybean transforma-tion efficiency, may be achieved by further optimizing
Phosphinothricin ppt
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WebKanamycin and phosphinothricin (PPT) resistance of T2-seedlings and copy number as determined by Southern blot Source publication Resveratrol glucoside (Piceid) synthesis … WebSep 15, 2014 · bar基 因编码对除草剂PPT(Phosphinothricin)和双丙氨磷(Bialaphos)的抗性,适合 用作禾谷类作物的转化体筛选。 在进行候选克隆的功能互补转化过程中,用bar基因筛选较困难,假阳性率 高。
WebShowing 1-1 of 1 result for "phosphinothricin" within Products. Products Genes Papers Technical Documents Site Content Chromatograms. Filter & Sort. All Photos (2) … WebJan 5, 2024 · 1. Introduction. L-Phosphinothricin (L-PPT; L-2-amino-4-(hydroxy(methyl)-phosphonoyl)butanoic acid) is the active ingredient of many commercial herbicides used worldwide such as Basta, Buster, Challenge, Finale, Harvest, and Ignite [1].As L-PPT resembles L-glutamic acid (L-Glu), it functions as a false substrate and irreversibly blocks …
WebApr 1, 2024 · L-phosphinothricin (L-PPT) is a competitive and environmentally friendly herbicide. To develop an efficient approach for synthesis of l-PPT, a kinetic resolution route with a novel... WebAbout 50 =B5M phosphinothricin (PPT, the active ingredient in Basta) is sufficient for the selection *** I used pure phosphinotricin for selection of BASTA resistant Arabidopsis seedlings harboring a p35S-bar gene. This worked nicely at a concentration of 0.05 mM.
WebPost sterilization, seeds were spread out onto plates that contained standard Arabidopsis growth medium (half-strength Murashige and Skoog (MS) salts) which was supplemented with the selective agent, phosphinothricin (PPT), at a concentration of 10 mg/L.
WebMar 9, 2024 · This chapter describes a protocol for protoplast transformation and plant regeneration for major forage and turf grass species, including tall fescue, red fescue, meadow fescue, perennial ryegrass, and Italian ryegrass. Embryogenic calli induced from caryopsis were used to establish embryogenic cell suspension cultures. easter lebanon 2023WebA glutamina sintetase ( GS) ( EC 6.3.1.2) [ 3] é un encima que xoga un papel esencial no metabolismo do nitróxeno catalizando a condensación do glutamato e o amoníaco para formar glutamina : Glutamato + ATP + NH 3 → Glutamina + ADP + fosfato. A glutamina sintetase usa o amoníaco producido pola redución de nitratos, degradación de ... cu denver school of nursing academic calendarWebApr 24, 2024 · Transaminase responsible for alienating prochiral ketone compound is applicable to asymmetric synthesis of herbicide L-phosphinothricin (L-PPT). In this work, the covalent immobilization of recombinant transaminase from Citrobacter koseri (CkTA) was investigated on different epoxy resins. easter leeds porcelain trivetsWebPublished: October 1999 A facile method for screening for phosphinothricin (PPT)-resistant transgenic wheats Sonriza Rasco-Gaunt, Amanda Riley, Paul Lazzeri & Pilar Barcelo … easter leaflets for childrenWebAug 16, 2007 · The present investigation describes a PPT-based selection system for alfalfa transformation that uses the phosphinothricin acetyl-transferase ( pat) gene as a selectable marker and 5.0 mg l −1 of bialaphos as the selective agent. easter legend of the dogwood treeWebSep 12, 2014 · 此可读框架通过组成型启动子CaMV35S启动,携带PPT乙酰基转移酶(PAT)基因作为选择标记基因,并将构建的表达载体导入根癌农杆菌EHA105。确定烟草转化的潮霉素临界筛选浓度为50mg,头孢噻肟钠的抑菌浓度为200mg。 cu denver softwareWebThe present invention relates to a method of Agrobaterium-based transformation of a plant comprising: generating a callus explant by, performing callus induction and maintenance in a plant in at least a first solution comprising less than about 100 mg/l of inositol; subjecting the callus explant to a cold treatment in a second solution comprising glutamine; and co … cu denver teaching assistant