Fathead minnow fhm cells

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WebJun 21, 2006 · The fathead minnow (FHM) is widely used as a standard test species for acute and chronic toxicity testing of contaminants, effluents, and receiving waters. Because of its widespread distribution throughout North America, this species also has application in monitoring studies and for deployment using caged exposures in the field. However, the … Webonto a preformed monolayer of Vero cells. In 2001, fathead minnow (FHM), white sturgeon skin (WSS), epithelioma papillosum caprini (EPC), and channel catfish ovary (CCO) cells were used instead of the Vero cells. Inoculated cells were incubated in a 5% CO2 atmosphere at 37°C. Cells were examined each day for viral cytopathic effect (CPE).

4.2 Primary diagnoses in fathead minnow, Japanese medaka …

WebJun 7, 2024 · 46 apoptosis in transfected fathead minnow (FHM) cells was investigated. Here, we confirmed that 47 ORF3141 was a secreted protein that targeted host cell mitochondria and induced cell apoptosis in WebSep 21, 2024 · In order to determine the subcellular localization and study the preliminary function of SOD from N. seriolae (NsSOD), gene cloning, secreted protein identification, … up da holler pizza chelyan wv

Digital Atlas of Fathead Minnow Histology: …

WebIncreased cells (spermatogonia) in fathead minnow testis: (a) Testis from adult male FHM negative control. (b) Spermatogonia dominate the germinal epithelium in this testis from adult male FHM exposed to 10 nM 17ß -estradiol for 10 days. Other diagnoses for this section include “decreased cells, spermatocytes” and “decreased cells ... Web@article{osti_5427784, title = {Fathead minnow FHM cells for use in in vitro cytotoxicity assays of aquatic pollutants}, author = {Babich, H and Borenfreund, E}, abstractNote = … WebAug 1, 1987 · Overall, the FHM cells were more sensitive than were the BF-2 cells. However, there was a better correlation between the in vitro cytotoxicity data for the BF-2 cell culture and LC 50 data for bluegill sunfish than between similar data for the FHM cell line and fathead minnows. recurdyn crack

Advancing the fathead minnow (Pimephales promelas) as a …

Isolation and characterization of plasma-derived …

WebJournal: Virology Journal Article Title: Expression of frog virus 3 genes is impaired in mammalian cell lines doi: 10.1186/1743-422X-5-83 Figure Lengend Snippet: FV3 … WebThe liver is a largest of the extramural organs. It is roughly U-shaped, situated ventral to the esophagus and conforming to the peritoneal cavity and surrounding viscera. The color varies from dark brown to cream or even yellow. Functions of the liver include assimilation of nutrients, production of bile, detoxification, hematopoiesis, and ... recur camberleyWebJun 6, 2007 · A fish cell line, fathead minnow (FHM) cell, was used to investigate the alteration of mitochondrial dynamics and the mechanism of apoptosis under Rana grylio … recup waregem

"WebApr 13, 2024 · 细胞核萎缩、核染色质边缘化，线粒体肿胀、坏死、嵴断裂，细胞质空泡化。取患病鲫组织匀浆滤菌后接种到鲤上皮瘤细胞系 (epithelioma papulosum cyprinid cell line，EPC) 和胖头鲤肌肉细胞系 (fathead minnow cell line, FHM)，盲传 3 代后未见细胞病 … " - Fathead minnow fhm cells

Fathead minnow fhm cells

PXD010913 - Integrated transcriptomics and proteomics analyses …

WebAug 1, 2024 · The general approach taken in these studies has involved exposing fathead minnows to a contaminant of interest, followed by some assessment of immunity, often immune-related gene expression or immune cell function, in the absence of in vivo immunological stimuli (e.g., pathogens, mitogens, etc.). While these studies provide … WebApr 13, 2011 · Therefore, we used the RTG-2 cell line to isolate IPNV and it is susceptible to produce CPE than epithelioma papulosu cyprini (EPC) and fathead minnow epithelial (FHM) cell line (Kelly et al ...

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WebAbstract. The viral susceptibility range of a poikilothermic cell line derived from the fathead minnow (Pimephales promelas) (FHM) to infection by a number of homoiothermic … WebABSTRACT: Invasion of Edwardsiella ictaluri into cultured mammalian, fish and enzymatically harvested catfish enteric epithelial cells is described. Gentamicin survival assays were used to demonstrate the ability of this catfish pathogen to invade IEC-6 (origin: rat small intestinal epithelium), Henle 407 (origin: human embryonic intestinal …

WebAug 23, 2016 · The present study utilizes the OECD 210 Early-life Stage (ELS) study to investigate the impacts levonorgestrel (LNG), a synthetic progestin, on fathead minnow (FHM) survival and growth. WebMar 11, 2024 · In fish cells, there are a handful of carefully controlled experiments with convincing evidence of gene knockdown driven by shRNA-produced by a plasmid. Xie et al. provided convincing data on the fathead minnow (FHM) cell line at 29°C by using co-transfection experiment with a lacZ- and corresponding shRNA-expressing plasmids. …

WebAug 1, 1987 · CONCLUSION The fathead minnow FHM cell line was suitable for use in the neutral red, cell protein, and cell detachment assays, but not in a colony-formation … WebINIS Repository Search provides online access to one of the world's largest collections on the peaceful uses of nuclear science and technology. The International Nuclear Information System is operated by the IAEA in collaboration with over 150 members.

Web（ Oncorhynchus mykiss）性腺细胞系（ RTG － 2 ，是被公认的第一株真骨鱼类的永生性细胞系。随后 Gravell [4 ] Maisberger 1965 和于建立了另一株鱼类细胞系—胖头鲤（ Fathead minnow ）肌肉细胞系（ FHM）。鱼类细胞系的建立表明鱼类体外培养技术的成 …

WebDec 8, 2015 · This study was conducted to investigate the protective effect of L‐carnitine (LC) against H 2 O 2 ‐induced oxidative stress in the fathead minnow muscle cell line (FHM). The FHM cells were stimulated with 1 mM H 2 O 2 for 1 h after LC pre‐treatment, and the cell viability and the activity and mRNA relative expression of antioxidant … recurdyn 2023 torrentWebThe Fathead Minnow was collected in 926 bioassessment sampling sessions and 147 fisheries assessment sessions. It was present in 96 rapid bioassessment sessions and … recura mvz adlershofWebFathead minnows are found in every drainage in Minnesota. It is the most common species of minnow in the state. They live in many kinds of lakes and streams, but are especially … recur chrgs stmt creditWebSep 21, 2024 · In order to determine the subcellular localization and study the preliminary function of SOD from N. seriolae (NsSOD), gene cloning, secreted protein identification, subcellular localization in fish cells, and apoptosis detection of NsSOD were carried out in this study. Subcellular localization research revealed that NsSOD-GFP fusion proteins ... update 0.5 tower defense: shinobiWebFathead Minnows. Fathead minnows are a common source of additional food for fish. Fatheads can tolerate lower oxygen levels than most game fish. They have a short life … recur as arthritis crossword clueWebThe samples were inoculated on to cells of the fathead minnow cell line (FHM), incubated at 30 degrees C and observed for 10 days. Cytopathic effect was noted. Medium from cultures were then passaged onto a new cultures for a blind passage (on cultures that had no cytopathic effect) and to confirm infectivity (on cultures that showed cytopathic ... recurbrowseWebThe integumentary system or skin of the fathead minnow is composed of two layers, an outer epidermis and an underlying dermis (Fig. 1). The epidermis is additionally divided into an outer fusiform layer composed of … recurdyn amesim